Analytical method development and validation analysis for quantitative assessment of cypermethrin by HPLC procedure
نویسندگان
چکیده
The precise, systematic, definite, particular, linear, exact and robust scientific method was developed validated for the assay of Cypermethrin in 25% EC Insecticide Injection, USP 500 mg/vial. Presently utilized 25 % as a working standard having limit 95% development validation. quantitative determination bring to accomplished by HPLC- Waters - Alliance 510 system equipped with UV/PDA detector. Methanol, Acetonitrile water ratio (60:20:20 v/v/v) used mobile phase flow rate 1.0 ml / min. 20 minutes run time. detection carried at 225 nm Nucleosil C18 column (250 mm × 4.6 x 10 µm) ambient temperature maintained. In this connection, uses μl injection volume diluent blank solution. linearity found be linear range 50% 150% concentration analytical is ppm 75 ppm. accuracy precision were within acceptable. present HPLC suitable. solution stable up 48 Hrs room temperature.
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ژورنال
عنوان ژورنال: World Journal Of Advanced Research and Reviews
سال: 2022
ISSN: ['2581-9615']
DOI: https://doi.org/10.30574/wjarr.2022.14.1.0352